Therefore, the lipid components of plasma, ovules, and semen (total lipid, lipid classes, and fatty acids) were analyzed in this study.
Methods and Materials
Two practical diets were formulated for characid broodstock with ingredients commonly used in fish diets in Iquitos. Palm oil (4%) was added to each diet to increase the
available energy and alpha-tocopherol levels. Palm oil has been used successfully in Colossoma diets previously (Viegas and Guzman, 1998). We determined that pijuayo and corn are similar in proximate composition. Due to the additional
Table 1. Composition (%) of diets for characid broodstock.a
Table 2. Calculated lipid and fatty acid composition (%) of diets for characid broodstock.
Table 3. Calculated essential amino acid composition (%) of diets for characid broodstock.
Table 4. Analyzed total lipid (% of tissue), lipid classes (g/100g lipid), and selected fatty acids (g/100g lipid) of plasma of Colossoma macropomum (gamitano) broodstock fed diets differing in protein content.a
a. The calculated energy to protein ratios of the diets with 40 and 30% protein are 7.3 and 8.5 kcal/gram dietary protein, respectively, based on digestible energy data for paco reported in Fernandes et al. The values are 8.2 and 10.1 kcal/gram dietary protein, respectively, based on digestible energy values for channel catfish or physiological fuel values (NRC 1993).
b. Total dietary protein levels calculated using analyzed protein content of the individual ingredients.
c. The diet with 40% pijuayo had 6% pijuayo and the diet with 30% protein had 10% pijuayo and 6% corn.
a. The calculated total lipid of diets with 40% and 30% protein was 7.8 and 8.0 %, respectively.
a. The total amount of these amino acids is given due to the ability of the non-essential amino acid in each pair (listed second) to partly fill the requirement for the essential amino acid.
a. Values are means + SE of 4 individual fish per diet. There were no significant differences between treatments as determined by ANOVA (P>0.05).
