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| Figure 1. Effect on masculinization of a single immersion of Oreochromis niloticus fry in trenbolone acetate (TA) on 12, 13, or 14 days post-fertilization. Figure depicts mean + SE percentage of males in each treatment with n = 99–101 per treatment (pooled triplicates). Fish were immersed for 3 h at a density of 33 fish l-1. Statistically significant differences from controls are represented by asterisks (*** P < 0.001). |